Journal: Molecular & Cellular Proteomics

Article Title: Elongation of Axons during Regeneration Involves Retinal Crystallin β b2 (crybb2)

doi: 10.1074/mcp.m600245-mcp200

Figure Lengend Snippet: FIG. 8. Double labeling immunofluo- rescence of RGC-5 cells. A–C, colocal- ization of crybb with calmodulin (calmod.) in particular along the filopo- dial processes. D–F, colocalization of crybb with synaptotagmin (syntaptot.) in the cell body but not along the cell pro- cesses. G–I, images showing that tau-1 is mainly localized in the cell body and partially within one process (arrow), whereas -crystallin outlines all cell pro- cesses. Scale bar, 10 m.

Article Snippet: Calmodulin, synaptotagmin, and tau-1 were detected with sheep antiserum (Chemicon, Limburg, Germany; dilution, 1:400), goat antiserum (Santa Cruz Biotechnology, Heidelberg, Germany; dilution, 1:200), and monoclonal mouse antibody (Chemicon; dilution, 1:200), respectively.

Techniques: Labeling

Journal: International Journal of Molecular Sciences

Article Title: A Crosslinking Analysis of GAP-43 Interactions with Other Proteins in Differentiated N1E-115 Cells

doi: 10.3390/ijms9091753

Figure Lengend Snippet: Calmodulin, but not actin, co-immunoprecipitates with GAP-43 following DSP treatment of cells. (a, b): DMSO. (c, d): DSP. Anti-β-galactosidase (a, c). Monoclonal anti-GAP-43 (b, d). (e) The immunoprecipitation supernatant from (d) was treated with monoclonal anti-calmodulin. Exposure time = 2 days (a–d) and 4 days (e).

Article Snippet: Calmodulin Sepharose 4B was from Amersham Biosciences.

Techniques: Immunoprecipitation

Journal: International Journal of Molecular Sciences

Article Title: A Crosslinking Analysis of GAP-43 Interactions with Other Proteins in Differentiated N1E-115 Cells

doi: 10.3390/ijms9091753

Figure Lengend Snippet: Polyclonal anti-GAP-43 yields co-immunoprecipitated calmodulin but not actin. (a) Cells treated with DMSO. (b) Cells treated with DSP. Exposure time = 6 days.

Article Snippet: Calmodulin Sepharose 4B was from Amersham Biosciences.

Techniques: Immunoprecipitation

Journal: International Journal of Molecular Sciences

Article Title: A Crosslinking Analysis of GAP-43 Interactions with Other Proteins in Differentiated N1E-115 Cells

doi: 10.3390/ijms9091753

Figure Lengend Snippet: Quantitation of GAP-43 and Calmodulin in Immunoprecipitates.

Article Snippet: Calmodulin Sepharose 4B was from Amersham Biosciences.

Techniques: Quantitation Assay

Journal: bioRxiv

Article Title: Postnatal reduction of eIF4E overexpression in D1-SPNs ameliorates KCNQ dysfunction, hyperexcitability and ASD-like behaviours

doi: 10.1101/2025.01.18.633694

Figure Lengend Snippet: Novelty reveals increased activity in striatonigral SPNs of eIF4E-TG mice. a ) Coronal brain slice showing example of electrode placement within the dorsal striatum, with GCaMP7s expression shown in green, and DARPP32 immunostaining (indicating the striatum) in magenta. Scale bar indicates 1mm. b ) Schematic of the experimental protocol, with two days of habituation to an empty home cage (blue) followed by one day of recording, including initial habituation to home cage (blue) with patch cable, followed by baseline recording before transfer to novel environment (pink). c ) Averaged Z-score normalised GCaMP7s traces in D1-Cre/WT and -/TG mice (6 WT and 6 TG). Z-scores are calculated from the pre-novelty baseline activity. d ) Change in GCaMP7s activity, shown as a combined average of three distinct parameters relating to activity (Supplemental Fig. 2a, c and e>), expressed as a change from baseline, for the direct pathway SPNs (unpaired T-test: t=2.27, *p=0.0465). e ) Averaged Z-score normalised GCaMP7s traces, during the timepoint one minute before until one minute after introduction to novel environment, in A2a-Cre/WT and -/TG mice (8 WT and 6 TG). Z-scores are calculated from the pre-novelty baseline activity. f ) Change in GCaMP7s activity, shown as a combined average of three distinct parameters relating to activity (Supplemental Fig. 2b, d and f>), expressed as a change from baseline, for the indirect pathway SPNs. In all panels, data are presented as mean ± SEM, with dots representing individual values for each mouse. The mean is indicated by the bar height or connecting line, while the SEM is shown by error bars or lines with shading. For full details of statistical analysis including negative results, refer to supplemental table 1.

Article Snippet: Slices were washed in TBS and then incubated in primary antibody overnight at +4C (1:200 R&D system MAB3228 mouse anti- eIF4E, 1:500 Synaptic System 382004 guinea pig anti-DARPP32, 1:400 ImmunoStar 20065 rabbit anti-enkephalin, 1:2000 Abcam ab13970 chicken anti-GFP, 1:1000 Rockland 600-401- 379 rabbit anti-RFP, 1:500 Rockland 200-301-379 mouse anti-RFP).

Techniques: Activity Assay, Slice Preparation, Expressing, Immunostaining